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hi-5 cells (Thermo Fisher)

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Thermo Fisher hi-5 cells
Hi 5 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hi+5+cells/high+five+cells/pm39747483-354-0-4
Average 90 stars, based on 1 article reviews

hi-5 cells - by Bioz Stars, 2026-10

90/100 stars

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Cell Culture:

Article Title: Identifying small molecule probes of ENTPD5 through high throughput screening
Article Snippet: Baculuovirus stocks were amplified in SF9 cells (11496015 Gibco) cultured in Sf-900 II SFM (10902096 ThermoFisher). .. Six days after inoculation of 1 L of Hi-5 cells (B85502 ThermoFisher) cultured in Express Five SFM (10486025 ThermoFisher) with 100 mL of high-titer baculouvirus, the cells were centrifuged at 4,000 RPM for 25 minutes, the supernatant was filtered with a 0.22 μM filter and added to 5 mL of loose Ni-Sepharose Excel beads (17371201 GE Life Sciences). ..

Construct:

Article Title: Purification of an insect juvenile hormone receptor complex enables insights into its post-translational phosphorylation
Article Snippet: .. Sf9 -II, Sf9 -III, and Hi-5 cells (Life Technologies) were used for the production of receptor proteins from various constructs. .. Cells were incubated in E-flasks using serum-free media (SFM; Life Technologies), SF900-II SFM for Sf9 -II and Hi-5 cells or Sf900-III SFM for Sf9 -III cells, supplemented with 0.1% Pluronic (F68; Life Technologies) to minimize shear damage to cells.

Infection:

Article Title: Association of DNA Polymerase ? (pol ?) with Ku and Ligase IV: Role for pol ? in End-Joining Double-Strand Break Repair
Article Snippet: A viral isolate for expression of the 5′ His-TdT pFASTBAC1 construct was prepared by the “Bac-to-Bac” method (Invitrogen). .. A pellet of approximately 2.5 × 10 8 Hi-5 cells (Invitrogen) infected with the 5′ His-TdT baculovirus was extracted in a buffer containing 50 mM NaPO 4 (pH 8.0), 1 M KCl, 10% glycerol, 0.2% Triton X-100, 10 mM imidazole, 7 mM β-mercaptoethanol, 1 mM PMSF, 10 μM leupeptin, 1 μM pepstatin A, and 0.1 trypsin inhibitor unit (TIU) of aprotinin/ml. .. The extract was then clarified ( 20 ), loaded onto a Ni-NTA Superflow (Qiagen) column, washed with 4 column volumes of extraction buffer, and eluted with extraction buffer supplemented with 350 mM imidazole.

Article Title: Association of DNA Polymerase μ (pol μ) with Ku and Ligase IV: Role for pol μ in End-Joining Double-Strand Break Repair
Article Snippet: A viral isolate for expression of the 5 His-TdT pFASTBAC1 construct was prepared by the “Bac-to-Bac” method (Invitrogen). .. A pellet of approximately 2.5 108 Hi-5 cells (Invitrogen) infected with the 5 His-TdT baculovirus was extracted in a buffer containing 50 mM NaPO4 (pH 8.0), 1 M KCl, 10% glycerol, 0.2% Triton X-100, 10 mM imidazole, 7 mM -mercaptoethanol, 1 mM PMSF, 10 M leupeptin, 1 M pepstatin A, and 0.1 trypsin inhibitor unit (TIU) of aprotinin/ml. .. The extract was then clarified (20), loaded onto a Ni-NTA Superflow (Qiagen) column, washed with 4 column volumes of extraction buffer, and eluted with extraction buffer supplemented with 350 mM imidazole.